Journal: Journal of Cellular and Molecular Medicine

Article Title: High-mobility group box-1 induces vascular remodelling processes via c-Jun activation

doi: 10.1111/jcmm.12519

Figure Lengend Snippet: Human pulmonary arterial endothelial cells and smooth muscle cells express the HMGB1 receptors TLR4 and RAGE. Relative TLR4 and RAGE mRNA expression (A and B) and protein (C) expression in human pulmonary arterial endothelial cells (PAEC) and human pulmonary arterial smooth muscle cells (PASMC); D1–D3 represent samples from three different donors for PASMC or lots for PAEC.

Article Snippet: Primary human arterial endothelial cells (PAEC) were purchased from Lonza (Basel, Switzerland) ( n = 6), cultured in VascuLife® VEGF Medium (LifeLine Technology, Frederick, MD, USA) and used for experiments until passage 8.

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: High-mobility group box-1 induces vascular remodelling processes via c-Jun activation

doi: 10.1111/jcmm.12519

Figure Lengend Snippet: HMGB1-induced proliferation of pulmonary arterial endothelial cells and smooth muscle cells. Effect of HMGB1 treatment on human pulmonary arterial smooth muscle cells (PASMC, A–D) and human pulmonary arterial endothelial cells (PAEC, E–H) on (A and E) proliferation n = 5–6, (B and F) apoptosis n = 5, (C and G) attachment n = 5 and (D and H) migration n = 4–6. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Primary human arterial endothelial cells (PAEC) were purchased from Lonza (Basel, Switzerland) ( n = 6), cultured in VascuLife® VEGF Medium (LifeLine Technology, Frederick, MD, USA) and used for experiments until passage 8.

Techniques: Migration

Journal: Journal of Cellular and Molecular Medicine

Article Title: High-mobility group box-1 induces vascular remodelling processes via c-Jun activation

doi: 10.1111/jcmm.12519

Figure Lengend Snippet: HMGB1 activates MAPK intracellular signalling pathways. Western blot analysis for MAPK and downstream factor activation in (A) human pulmonary arterial smooth muscle cells (PASMC) and (B) human pulmonary arterial endothelial cells (PAEC) following stimulation with 1 or 100 ng/ml HMGB1 for the indicated time-points. Blots are representative of a minimum three independent experiments.

Article Snippet: Primary human arterial endothelial cells (PAEC) were purchased from Lonza (Basel, Switzerland) ( n = 6), cultured in VascuLife® VEGF Medium (LifeLine Technology, Frederick, MD, USA) and used for experiments until passage 8.

Techniques: Western Blot, Activation Assay

Journal: Frontiers in Genetics

Article Title: Mxi1-0 Promotes Hypoxic Pulmonary Hypertension Via ERK/c-Myc-dependent Proliferation of Arterial Smooth Muscle Cells

doi: 10.3389/fgene.2022.810157

Figure Lengend Snippet: Hypoxia induces expression of Mxi1-0 but not Mxi1-1 in PASMCs. (A) PASMCs were exposed to hypoxia (1% O 2 ) for indicated periods of time. Cell lysates were prepared and subjected to Western blotting assay. (B) Pulmonary arterial endothelial cells (PAECs) and PASMCs were exposed to normoxia (21% O 2 ) or hypoxia for 12 h, and were subjected to Western blottinganalyses. (C,D) PASMCs were transfected with constructs for HA-tagged Mxi1-0 (C) or Flag-tagged Mxi1-1 (D) , and were subjected to Western blotting analyses. (E) PASMCs were exposed to normoxia or hypoxia for 12 h, and were subjected to immunostaining with a Mxi1 antibody and counterstaining of the nuclei with DAPI. Scale bar, 20 μm. Data from three independent experiments are shown as means ± SDs. For statistical significance, *** represents p < 0.001 compared to normoxia or the mock-transfected group.

Article Snippet: Primary human PAECs were purchased from ScienCell, Inc (#3100) and cultured in endothelial cell medium (ECM, ScienCell).

Techniques: Expressing, Western Blot, Transfection, Construct, Immunostaining